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The antibody is from purified rabbit serum by affinity purification by using sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of the gene has become noticed in lots of tumors, which results in chromosome instability, and thus may perhaps lead to tumorigenesis. This protein was identified to associate with, and become associated with, the phosphorylation of NPAT protein (nuclear protein mapped towards the ATM locus), which participates in mobile-cycle controlled histone gene expression and plays a important position in marketing cell-cycle development inside the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode unique isoforms, happen to be explained. Two more splice variants ended up noted but detailed nucleotide sequence facts just isn't still accessible.
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Blocking peptides are peptides that bind exclusively towards the focus on antibody and block antibody binding. These peptide usually is made up of the epitope regarded from the antibody.
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This guidebook outlines more achievable optimization of mobile and immunoassay parameters to make certain the absolute best outcomes are...
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This tutorial outlines even more achievable optimization of cellular and immunoassay parameters to be certain the best possible outcomes are...
Antibodies bound to the blocking peptide no longer bind to the epitope over the target protein. By evaluating the staining within the blocked antibody vs . the antibody by itself, you can see which staining is distinct.
Focus on Facts Cyclin E belongs into the extremely conserved cyclin family, whose members are characterized by a spectacular periodicity in protein abundance with the mobile cycle. Cyclins functionality as regulators of CDK kinases. Various cyclins show distinct expression and degradation patterns which lead on the temporal coordination of each and every mitotic occasion. Cyclin E kinds a posh with and capabilities as being a regulatory subunit of CDK2, whose action is needed for mobile cycle G1/S transition. This protein accumulates on the G1-S period boundary which is degraded as cells development as a result of S section.
The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by equally antibodies...
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